Hello I encountered the following while running DADA2 in Qiime2 using this command: qiime dada2 denoise-paired --p-n-threads 8 --i-demultiplexe d-seqs p02_primer_trimmed.qza --p-trunc-len-f 258 --p-trunc-len-r 211 --output-d ir DADA2_denoising_output --verbose > out.log
This error encountered: Loading required package: Rcpp
Error: package or namespace load failed for ‘dada2’ in dyn.load(file, DLLpath = DLLpath, ...):
unable to load shared object '/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/en vs/qiime2-2020.2/lib/R/library/data.table/libs/datatable.so':
/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/R/librar y/data.table/libs/datatable.so: failed to map segment from shared object: Cannot allocate memory
Execution halted
Traceback (most recent call last):
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/site-packages/q2_dada2/_denoise.py", line 257, in denoise_paired
run_commands([cmd])
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands
subprocess.run(cmd, check=True)
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/subprocess.py", line 418, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_dada_paired.R', '/tmp/tmpesrr8g2n/ forward', '/tmp/tmpesrr8g2n/reverse', '/tmp/tmpesrr8g2n/output.tsv.biom', '/tmp/ tmpesrr8g2n/track.tsv', '/tmp/tmpesrr8g2n/filt_f', '/tmp/tmpesrr8g2n/filt_r', '2 58', '211', '0', '0', '2.0', '2.0', '2', 'consensus', '1.0', '8', '1000000']' re turned non-zero exit status 1.
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/site-packages/q2cli/commands.py", line 328, in call
results = action(**arguments)
File "</usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/p ython3.6/site-packages/decorator.py:decorator-gen-455>", line 2, in denoise_pair ed
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/site-packages/qiime2/sdk/action.py", line 245, in bound_callable
output_types, provenance)
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/site-packages/qiime2/sdk/action.py", line 390, in callable_executor
output_views = self._callable(**view_args)
File "/usr/site/hpc/x86_64/generic/Anaconda3/2020.03/envs/qiime2-2020.2/lib/py thon3.6/site-packages/q2_dada2/_denoise.py", line 272, in denoise_paired
" and stderr to learn more." % e.returncode)
Exception: An error was encountered while running DADA2 in R (return code 1), pl ease inspect stdout and stderr to learn more.
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please insp ect stdout and stderr to learn more.
See above for debug info.
I have already installed R and RStudio.
Thank you so much in advance.