Hi there! Trying to run my samples using QIIME2 2023.7. As a novice, cutadapt has never been an issue before. However, while running my samples today I encountered the following error:
The command used was:
qiime cutadapt trim-paired --i-demultiplexed-sequences paired-end-demux.qza --o-trimmed-sequences paired-end-trimmed.qza
Where could I have gone wrong? Quick help would really be appreciated!
Tried the same with QIIME2 2023.9 on AWS. Encountered the same error!
Welcome to the forums!
Thanks for posting your full error message. I notice both errors say
"debug info has been saved to..." a .log file!
Can you download and post that .log file here? You can also open it up and look for clues!
Hi! Here are snapshots of the log file:
That second screenshot looks good. Samples are being processed.
That first screenshot has an error. Do you see it?
Did you pre-process these reads with another program before using Qiime2? Maybe try downloading just that one file again to in case it was corrupted during downloading.
Thank you so much for your help! Turns out there was an error with six more files from the same bioproject - the reads don't match. Have proceeded without these reads for my analysis.
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