I am trying to apply the PICRUSt2 tutorial to my dataset, but it requires ".fna" file. I currently have 10
".fasta" files, shall I convert those file to 1 ".fna" and then run the analysis ? if yes, how? The link of the tutorial I am trying to apply is the following:
Please note that I am using QIIME2-2019.10 & PICRUSt 2 v2.4.2 conda.
Welcome to the forusm!
Good news! That's the same file type on the inside, just with a different file extension. You can use your .fasta file just like the .fna files are used in the tutorials. (You could also rename them to .fna if you wanted, but no conversion is needed.)
That tutorial you liked uses the PICRUSt2 pipeline independently of Qiime2. You could also take a look at the q2-picrust2 plugin for Qiime2 its tutorial.
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