Hie guys am trying thinking of doing predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. I'm hoping to do this using PICRUSt however during taxonomic classification i used SILVA as my reference database instead of Greengenes does this matter since PICRUSt s…

Hi @Tinaye , No your taxonomic reference database doesn't matter, because PICRUSt 2 uses it's own reference tree. I'd recommend reading the PICRUSt tutorial because it better explains better than I can. Best, Justine

Thanks hey however I,ve tried the q2-PICRUSt plugin installation and during the process i encounter this error (qiime2-2018.11) MacBooks-MBP:picrust2-2.0.3-b macbook$ conda update -n qiime2-2018.11 -f picrust2-env.yaml WARNING: The --force flag will be removed in a future conda release. See 'cond…

I think you should follow the q2-picrust2 install guide. https://library.qiime2.org/plugins/q2-picrust2/13/ wget https://github.com/picrust/picrust2/releases/download/v2.0.3-b/picrust2-2.0.3-b.zip unzip picrust2-2.0.3-b.zip cd picrust2-2.0.3-b conda-env update -n qiime2-2019.1 -f picrust2-env.y…

If you're using qiime2.2018.11, you should use the name qiime2-2018.11 instead of qiime2-2019.1.

Yes i did, actually i changed my qiime to 2019.1 but i still get the same error. From what i can tell the problem might be my picrust2-env.yaml is a read only file.

For that, you could maybe try re-downloading the file in a directory where you have re-write permissions, or modifying the permission on the file?

Hey thanks that's the exact install guide am following which is giving me multiple errors. For example the conda-env command is not a command.

Hi @Tinaye , What version of conda are you running? Does using "conda env" instead of "conda-env" make a difference? Gavin

Hi @Tinaye Did u get it to work? I wonder if you had the same issue that I had. We were working in what are called as singularity containers. And because this is a plugin we were told to the following (as boldfaced) before the actual script and it worked. module load singularity/3 export TZ='Paci…

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