I am facing difficulty in annotating the phylogenetic tree using ITOL. I am using 2019.7 version and I constructed tree using align-to-tree-mafft-fasttree: Build a phylogenetic tree using fasttree and mafft alignment pipeline. Then I used aligned sequence file generated from above pipeline to build phylogenetic tree using fasttree: Construct a phylogenetic tree with FastTree. method. I exported nwk file of the tree and visualized in ITOL. As ITOL can support qza file for annotation then I tried to annotate tree using taxonomy.qza and featuretable.qza files separately. But in both case ITOL is showing parsing error log as “couldn’t find ID … in the tree”. Thenafter, I filtered feature table using filter-table: Remove features from table if they’re not present in tree. method for annotating the tree but again same error was displayed. Please suggest me what is wrong with this method of annotation?
Thanks in anticipation.