PERMANOVA analysis interpretation

Hi everyone,

Could you help me interpret why are the p-values from my PERMANOVA overall results different from the pairwise results if the test statistic is the same?

!qiime diversity beta-group-significance
--i-distance-matrix bray_curtis_distance_matrix.qza
--m-metadata-file sample-metadata.tsv
--m-metadata-column age_solids_6
--o-visualization beta_diversity/permanova_results/BC-age-solids-cat-significance.qzv

Thank you!

Actually, since you have only 2 levels in chosen column, it is not necessary to run PERMANOVA in pairwise mode, though it not incorrect either. P-value in the upper table is P-value of omnibus test, which tests if there is a variation among all levels in the column. With only 2 levels, as in your case, you can report this P-value and ignore second table, in which each level is compared with each othet level. In that case p-values are FDR corrected and converted to q-values. They are equal between each other just bacause with 2 levels there is no correction needed. P-values from first and second table are different because PERMANOVA will yield slightly different results when running several times.



Thank you for your explanation!!
I understand now, I was thrown off because I had always seen the same p-value in the two tables.

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