I have some questions related to analyzing my data at the level of phylum (L5).
I would like to do a PCA using R on my dataset which I clean them manually. The dataset stored in csv format. How can I do that without using phyloseq function? Because I do not have access to the previous output of qiime analysis that has been done by someone else.
I brought a small part of the data. The data format is like the following:
Hi @ari_sh70,
It sounds like you just want to make PCoA plots in R using data arrived from QIIME 2.
If you had access to a beta diversity matrix in QIIME 2, I would recommend following this tutorial.
The data table you show is basically a normal site x species table (if you subtract column 2:3, and assuming column 1 is your sample-ids) that you can use to make PCoA plots in R. There are no shortage of tutorials on this topic around the web. For example first one I saw was here. I did notice however that your table appears to be in relative abundances, which is not how I would recommend making your PCoA plots. So hopefully you can retrieve the original table...
Hi Mehrbod,
Thanks so very much for helping me out.
Yes, the table is in relative abundances.
Although, I made some PCA plot this afternoon but I need to digging into the links you've provided here and I hope I can find out more.