Hi
I am attending the Qiime2 workshop (Parkinson tutorial), but I got stuck while doing the taxonomy barchart section:
I ran the first command to obtain the Output artifacts: table_2k.qza (DONE correctly I got the qza file)
here is the problem
the second command to obtain the visualization: taxa_barplot.qzv as the output, I got the following message:
(1/1) Invalid value for '--i-taxonomy': './taxonomy.qza' is not a QIIME 2
** Artifact (.qza)**
I copied directly from tutorial so I do not know what happened I got this:
$ qiime taxa barplot \
--i-table ./table_2k.qza
--i-taxonomy ./taxonomy.qza
--m-metadata-file ./metadata.tsv
--o-visualization ./taxa_barplot.qzv
Usage: qiime taxa barplot [OPTIONS]
This visualizer produces an interactive barplot visualization of
taxonomies. Interactive features include multi-level sorting, plot
recoloring, sample relabeling, and SVG figure export.
Inputs:
--i-table ARTIFACT FeatureTable[Frequency]
Feature table to visualize at various taxonomic
levels. [required]
--i-taxonomy ARTIFACT FeatureData[Taxonomy]
Taxonomic annotations for features in the provided
feature table. All features in the feature table must
have a corresponding taxonomic annotation. Taxonomic
annotations that are not present in the feature table
will be ignored. [required]
Parameters:
--m-metadata-file METADATA...
(multiple The sample metadata.
arguments will be
merged) [required]
Outputs:
--o-visualization VISUALIZATION
[required]
Miscellaneous:
--output-dir PATH Output unspecified results to a directory
--verbose / --quiet Display verbose output to stdout and/or stderr
during execution of this action. Or silence output if
execution is successful (silence is golden).
--examples Show usage examples and exit.
--citations Show citations and exit.
--help Show this message and exit.
**There was a problem with the command: **
** (1/1) Invalid value for '--i-taxonomy': './taxonomy.qza' is not a QIIME 2**
** Artifact (.qza)**
(qiime2-2020.8)
Thanks in advance
forum!