OTU clustering cannot accept paired-end sequences as input

[colinbrislawn (Colin J Brislawn)]

Yes it is possible. It is discussed at length in this thread:

To cluster or not to cluster?

Hello - I would like to bring up a topic that has been discussed before (A quick clarification on clustering) and provide an example from my own analysis. I am looking at one particular taxonomic group within environmental samples of bacterial populations. The method of denoising is dada2, so I am assuming that OTU clustering was not necessary since sequencing errors are accounted for during the denoising process. However, this analysis resulted in many OTU's being defined, some of them quite r…