I want to do my analysis based on OTUs (Vesearch Plugin). To do this, I used demux as an input to dereplicate the sequences but it gave the error below.
There was a problem with the command:
(1/1) Invalid value for '--i-sequences': Expected an artifact of at least
type SampleData[Sequences] | SampleData[SequencesWithQuality] |
SampleData[JoinedSequencesWithQuality]. An artifact of type
SampleData[PairedEndSequencesWithQuality] was provided.
Not at all. As I told at first, I used demux file (not rep-seq file provided from DADA2).
Hopefully, I solved the error with your help.
I am asking a relevant question in this regard.
I have merged some groups table and sequences. I need to know is it possible I use them in vesearch to convert them to OTUs? If I want to start from scrach I have to spend more time that is why I am asking if there is a one way/shortcut then I do that to produce OTUs from ASVs!
Thanks a lot