Odd PCoA results based bray-curtis distance

Dear Friends,

I ran qiime2 analysis based on Deblur method. I took the bray curtis and unweighted UniFrac distance analysis, and found that the PCoA plot based bray-curtis distance had an odd sample distribution, that all the samples were in the lines. However, the unweighted unifrac distance method had a normal PCoA results.

You appear to have the same issue as this post:

I.E. for one reason or another there are very few shared ASVs between your samples. It is most likely a technical artifact related to some sort of processing or batch effect but could be biologically possible under some circumstances if you would have good reason to think there would literally be no shared organisms between communities.


Thanks! I found the reason that different primer lengths among these samples influenced the performance of Deblur.