In output FeatureTable table.qzv in Interactive Sample Detail is 'Sample ID' and 'Feature count'. Does 'Feature count' mean ASVs count in certain sample here?
In one of DADA2 output SampleData[DADA2Stats] I have table with sample-id, input, filtered... non-chimeric etc. And 'non-chimeric' value is the same as 'Feature count' mentioned earlier.
I assumed that non-chimeric reads are "good reds" that are used further in the analysis.. Could anyone explain me what exactly is mean by 'Feature Count' ?
Welcome to the forum!
Great question - feature count can refer to the total number of features observed, or the number of times a specific feature was observed (within a given sample, etc). I would advise against referring to ASVs and features interchangeably however - @Nicholas_Bokulich has a very concise description of the difference between ASVs and features, which you can find here (with further explanation on the general definition of a feature here).
A chimeric read (or split read) means that multiple subsections of a given read align to multiple distinct portions of the genome with little or no overlap. Non-chimeric reads refer to any read that aligns with a continuous portion of the genome (i.e. a 'real' segment of DNA that exists in the wild).
Hope this helps! Cheers
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