Hi,
I have Paired end data. However there is not overlap between my R1 and R2 reads. I has been reading and the solution when there is not overlapping could be to work just with R1 reads and avoid the merging step. However I could not import the data into qiime since they are reads from a paired end sequencing. there is any suggestion about how the manifest file should looks like and the parameters for importing should looks like for this case.
qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path manifest.csv
--output-path paired-end-demux.qza
--input-format PairedEndFastqManifestPhred33
Thanks in advance,