no overlapping reads, import just R1 read data in qiime

Hi,
I have Paired end data. However there is not overlap between my R1 and R2 reads. I has been reading and the solution when there is not overlapping could be to work just with R1 reads and avoid the merging step. However I could not import the data into qiime since they are reads from a paired end sequencing. there is any suggestion about how the manifest file should looks like and the parameters for importing should looks like for this case.

qiime tools import
--type 'SampleData[PairedEndSequencesWithQuality]'
--input-path manifest.csv
--output-path paired-end-demux.qza
--input-format PairedEndFastqManifestPhred33

Thanks in advance,

Hi @nana, welcome to :qiime2:!

You can follow the procedure outlined here.

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