I was working my way through the moving pictures tutorial, and there was a odd issue.
When running the demux command, I got this error:
There was a problem with the command:
(1/1) Invalid value for “–m-barcodes-file”: There was an issue with
retrieving column ‘barcodesequence’ from the metadata:
I downloaded the sample metadata file from here: https://data.qiime2.org/2020.2/tutorials/moving-pictures/sample_metadata.tsv
Upon inspecting the TSV file, I found that the second column is labeled as barcode-sequence and not barcodesequence. So I manually changed that column name and it ran fine. It just seemed like an odd thing to have to fix when working through a tutorial, so I wanted to at least check to make sure there wasn’t something else going on that I missed.
I am running QIIME2 on a Windows VirtualBox, QIIME 2 Core-2020.2.