Moving pictures - feature table and feature data summaries returning strange error codes

Technical Support
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1

Hello all, I’m running Qiime2 version 2024.10 in conda at my lab. They set me up going through the tutorial and I ran into an error - is it because of the versioning?

Command:

qiime feature-table summarize
--i-table table.qza
--m-metadata-file sample-metadata.tsv
--o-summary table.qzv
--o-feature-frequencies feature-frequencies.qza
--o-sample-frequencies sample-frequencies.qza
qiime feature-table tabulate-seqs
--i-data rep-seqs.qza
--o-visualization rep-seqs.qzv

The errors I get are

(1/4?) No such option: --m-metadata-file Did you mean --m-sample-metadata-file?
(2/4?) No such option: --o-summary
(3/4?) No such option: --o-feature-frequencies
(4/4?) No such option: --o-sample-frequencies

It did at least not return an error when I tried the suggested command of –m-sample-metadata-file but I suspect it did not do what I needed. Any help is greatly appreciated!

2

Hi @pants,

Welcome to the :qiime2: forum :waving_hand:

Yes, it looks like you're using the latest version of our user documentation (Moving Pictures tutorial 🎥 - Microbiome marker gene analysis with QIIME 2) but are working with QIIME 2 2024.10, which has some parameter differences in feature-table summarize.

Is there a reason your lab set you up with 2024.10? Is this the version that's been installed on the HPC, or is this on your personal machine? I would recommend upgrading to our latest version if possible, so that you can access the latest updates and new features available in 2026.1.

That being said, if this isn't an option- you can refer to our documentation from 2024.10, which will align with the version of QIIME 2 you're using.

Hope this helps!

Cheers :lizard:

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