Merged Reads fastq files from DADA2

Hi all, hoping somebody can help me with this.

Is there a way to get the fastq files for the merged reads that are joined after running DADA2? I followed the moving pictures tutorial. I'd like to take these joined reads and look at their quality on a different platform.
From what I can tell, DADA2 merges the reads when it is denoising them, but I can't figure out a way to export them after the fact.

Please read the following before posting!

Is this post about a User Support Question? Those include questions about specific results while running QIIME 2, warnings observed while running a QIIME 2 command. Please do not post questions here that have to do with interpretation of results, general discussion, or technical support.

Before posting, please make sure you have the following information available, in order for us to help you in a timely manner:

  • Have you searched for the problem on the forum? It is rare that we see a new question asked, so make sure you do your homework before asking for us to commit our time to helping you.
  • Have you reviewed the QIIME 2 Forum Glossary?
  • Version of QIIME 2 you are running, and how it is installed (e.g. Virtualbox, conda, etc.)
  • What is the exact command or commands you ran? Copy and paste please.
  • What is the exact error message, if applicable? If you didn't run the command with the --verbose flag, please re-run and copy-and-paste the results.

Hi @Anthony_DeSana! Welcome to the QIIME 2 forum!

The merged ASVs are the --o-representative-sequences Artifact, returned by dada2 denoise-paired. You can work with that FeatureData[Sequence] in many subsequent steps, and if you want to generate a table of the ASV sequences, run the following command:

https://docs.qiime2.org/2020.6/plugins/available/feature-table/tabulate-seqs/

All of this is covered in more detail in the Moving Pictures tutorial:

https://docs.qiime2.org/2020.6/tutorials/moving-pictures/

Have fun! :qiime2:

This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.