Memory Error- Demux Qiime2 2019.4

Technical Support
1

Hi everyone!

After searching for my issues on the forum, I believe I'm actually stuck. I keep getting a memory error message while I'm trying to run demux! I'm currently running Q2 2019.4 through VirtualBox on a 32GB HP. I'm trying to demultiplex my EMP-Single-End sequences [about 275 MB] and I keep getting an error message: "Memory Error". I ran the following command:

qiime demux emp-single --i-seqs '/home/qiime2/Documents/Kaylee/2017Data/Qiimedocs/empsingleend.qza' --m-barcodes-file '/home/qiime2/Documents/Kaylee/2017Data/qiime2/sample-metadata.tsv' --m-barcodes-column BarcodeSequence --p-no-golay-error-correction --p-no-rev-comp-mapping-barcodes --o-per-sample-sequences '/home/qiime2/Documents/Kaylee/2017Data/Qiimedocs/EMPdemultiplexed.qza' --o-error-correction-details '/home/qiime2/Documents/Kaylee/2017Data/Qiimedocs/empcorrectiondetails.qza'

I keep getting this error message, even after removing some of the parameters. (see below)

demuxerror
demuxerror1920×1080 257 KB

Any help would be greatly appreciated!!

Thank you!
Kaylee

2

You need to allocate more resources to VirtualBox. The VirtualBox manual should guide you on how you can do this. I am guessing the defaults are probably something like 1GB of memory, because demux is usually not a very memory-intensive action. Allocate as much memory as you can and let us know how that goes!

3

Thank you for the reply!! I’ll be working on my VM today and I’ll keep ya updatedc! thanks!

4

It worked!! Thank you so much!

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