Hi, thanks for your reply.
I have read your discussion and there are some points that I did not understand. I have been using a QIIME 2 image (version 2022.8) provided by others, which does not directly support your pre-trained UNITE 10 classifier (need QIIME2 version 2024.2) . Therefore, I used the latest data from the UNITE, importing sequences and taxonomy with 'qiime tools import', and trained the classifier using 'qiime feature-classifier fit-classifier-naive-bayes'. The classification results were slightly richer than before.
Does the discussion in this post indicate that different versions of QIIME 2 does not significantly affect species classification?