Hello, I used the ITS5 data and UNITE database to do the analysis but get many unclassified taxa. Here are the steps I took. The sequencing company provides the following data, one of which has undergone quality control, although the quality of the middle section is still very poor. [1] Another…

many unassigned fungal sequences

colinbrislawn (Colin J Brislawn) April 8, 2024, 8:49pm 6

Someone else was having problems with the unite-ver9-99-classifier-25.07.2023.qza. You could try the Unite v10 pretrained which just came out.