Am running 16S analysis for V4 region of drosophila microbiome and getting low non chimera between 40-50%. Could someone help out. Although I discovered that my reverse reads were bad
qiime dada2 denoise-paired
--i-demultiplexed-seqs demux-paired-end.qza
--p-trim-left-f 5
--p-trunc-len-f 249
--p-trim-left-r 4
--p-trunc-len-r 165
--o-representative-sequences rep-seqs-dada2.qza
--o-table table-dada2.qza
--o-denoising-stats stats-dada2.qza
stats-dada2.qzv (1.2 MB)