Hi!
I'm trying to analyze taxonomy assignment using long read sequencing data generated from Illumin HiSeq, PacBio, and Nanopore.
My workflow gonna be like this, roughly:
Import - dada2(single) - taxonomy assign.
This is my first time using long read sequencing data, what I'm wondering is,
which is appropriate position for dada2 to truncate the data?
When I use amplicon sequences, I made position where 25%-tile goes under Q20 first, but it does not seems to apply at long read.
Any suggestions will be appreciate.
Thank you!
EY