Many thanks for your kind response @SoilRotifer.
The good news is it did work, and taxonomy was added to the file, but it changed the OTU names from features ids to reference database ids, as highlighted in the below image, can I retain the feature ids as such?
Also I'm confused at the step where adding taxonomy (in step: biom add-metadata -i feature-table.biom -o feature-table-tax.biom --observation-metadata-fp 97_otu_taxonomy.txt --sc-separated taxonomy) to feature-table.biom, do I need to add reference taxonomy or taxonomy file generated after alignment of seqs-dada2?
Thank you so much
