LEfSe after QIIME2 to test at all taxonomic levels

@MiriamGorostidi,

First of all, it seems like you were able to run lefse-plot_res.py without any issues at least and the error is coming from lefse-plot_cladogram.py. Can you confirm this? For example, can you show me the output/Useful-Information/output.pdf file?

Secondly, you may have one or more outdated libraries installed in your LEfSe environment. Can you show me the result of conda list after activating your LEfSe environment?

(lefse) sbslee@x86_64-apple-darwin13 ~ % conda list
# packages in environment at /Users/sbslee/opt/anaconda3/envs/lefse:
#
# Name                    Version                   Build  Channel
_r-mutex                  1.0.1               anacondar_1    conda-forge
backports                 1.0                        py_2    conda-forge
backports.functools_lru_cache 1.6.1                      py_0    conda-forge
backports_abc             0.5                        py_1    conda-forge
biom-format               2.1.7                    py27_0    bioconda
bwidget                   1.9.14               h694c41f_0    conda-forge
bzip2                     1.0.8                hc929b4f_4    conda-forge
ca-certificates           2020.12.5            h033912b_0    conda-forge
cairo                     1.16.0            h0ab9d94_1001    conda-forge
cctools_osx-64            949.0.1             h2f0f38f_19    conda-forge
certifi                   2019.11.28       py27h8c360ce_1    conda-forge
clang                     11.0.1               h694c41f_1    conda-forge
clang-11                  11.0.1          default_hf8bb9ca_1    conda-forge
clang_osx-64              11.0.1               hb91bd55_0    conda-forge
clangxx                   11.0.1          default_hf8bb9ca_1    conda-forge
clangxx_osx-64            11.0.1               h7e1b574_0    conda-forge
click                     7.1.2              pyh9f0ad1d_0    conda-forge
compiler-rt               11.0.1               h654b07c_0    conda-forge
compiler-rt_osx-64        11.0.1               h8c5fa43_0    conda-forge
curl                      7.68.0               h8754def_0    conda-forge
cycler                    0.10.0                     py_2    conda-forge
fontconfig                2.13.1            h1027ab8_1000    conda-forge
freetype                  2.10.4               h4cff582_1    conda-forge
fribidi                   1.0.10               hbcb3906_0    conda-forge
functools32               3.2.3.2                    py_3    conda-forge
future                    0.18.2           py27h8c360ce_1    conda-forge
futures                   3.3.0            py27h8c360ce_1    conda-forge
gettext                   0.19.8.1          haf92f58_1004    conda-forge
gfortran_osx-64           4.8.5                h22b1bf0_8    conda-forge
glib                      2.66.3               h519c658_0    conda-forge
graphite2                 1.3.13            h2e338ed_1001    conda-forge
gsl                       2.4               ha2d443c_1006    conda-forge
h5py                      2.10.0          nompi_py27h106b333_102    conda-forge
harfbuzz                  2.4.0                h92b87b8_1    conda-forge
hdf5                      1.10.5          nompi_h0cbb7df_1103    conda-forge
icu                       58.2              h0a44026_1000    conda-forge
jpeg                      9d                   hbcb3906_0    conda-forge
kiwisolver                1.1.0            py27h5cd23e5_1    conda-forge
krb5                      1.16.4               h1752a42_0    conda-forge
ld64_osx-64               530                 hea264c1_19    conda-forge
ldid                      2.1.2                h7660a38_2    conda-forge
lefse                     1.0.8.post1              py27_2    bioconda
libblas                   3.8.0               14_openblas    conda-forge
libcblas                  3.8.0               14_openblas    conda-forge
libclang-cpp11            11.0.1          default_hf8bb9ca_1    conda-forge
libcurl                   7.68.0               h709d2b2_0    conda-forge
libcxx                    11.0.1               habf9029_0    conda-forge
libedit                   3.1.20191231         h0678c8f_2    conda-forge
libffi                    3.2.1             hb1e8313_1007    conda-forge
libgfortran               3.0.1                         0    conda-forge
libglib                   2.66.3               h2575888_0    conda-forge
libiconv                  1.16                 haf1e3a3_0    conda-forge
liblapack                 3.8.0               14_openblas    conda-forge
libllvm11                 11.0.1               h223d4b2_0    conda-forge
libopenblas               0.3.7                hd44dcd8_1    conda-forge
libpng                    1.6.37               h7cec526_2    conda-forge
libssh2                   1.9.0                h8a08a2b_5    conda-forge
libtiff                   4.2.0                h355d032_0    conda-forge
libwebp-base              1.1.0                hbcb3906_3    conda-forge
libxml2                   2.9.9                hd80cff7_2    conda-forge
linecache2                1.0.0                      py_1    conda-forge
llvm-openmp               11.0.1               h7c73e74_0    conda-forge
llvm-tools                11.0.1               h223d4b2_0    conda-forge
lz4-c                     1.9.3                h046ec9c_0    conda-forge
make                      4.3                  h22f3db7_1    conda-forge
matplotlib                2.1.2                    py27_1    conda-forge
matplotlib-base           2.1.2            py27h31f9439_1    conda-forge
ncurses                   6.2                  h2e338ed_4    conda-forge
numpy                     1.16.5           py27hde6bac1_0    conda-forge
openssl                   1.1.1i               h35c211d_0    conda-forge
pandas                    0.24.2           py27h86efe34_0    conda-forge
pango                     1.42.4               haa940fe_4    conda-forge
pcre                      8.44                 hb1e8313_0    conda-forge
pip                       20.1.1             pyh9f0ad1d_0    conda-forge
pixman                    0.38.0            h01d97ff_1003    conda-forge
pyparsing                 2.4.7              pyh9f0ad1d_0    conda-forge
pyqi                      0.3.2                    py27_1    bioconda
python                    2.7.15          h8e446fc_1011_cpython    conda-forge
python-dateutil           2.8.1                      py_0    conda-forge
python_abi                2.7                     1_cp27m    conda-forge
pytz                      2020.1             pyh9f0ad1d_0    conda-forge
r-base                    3.5.3                hb1347aa_0  
r-codetools               0.2_16          r35h6115d3f_1001    conda-forge
r-coin                    1.3_1             r35h159158b_0    conda-forge
r-lattice                 0.20_41           r35h17f1fa6_1    conda-forge
r-libcoin                 1.0_5             r35h26f5615_1    conda-forge
r-mass                    7.3_51.6          r35h17f1fa6_1    conda-forge
r-matrix                  1.2_18            r35h26f5615_2    conda-forge
r-matrixstats             0.56.0            r35h17f1fa6_0    conda-forge
r-modeltools              0.2_23            r35h6115d3f_0    conda-forge
r-multcomp                1.4_13            r35h6115d3f_0    conda-forge
r-mvtnorm                 1.0_11            r35haf69682_2    conda-forge
r-sandwich                2.5_1             r35h6115d3f_1    conda-forge
r-survival                3.1_12            r35h17f1fa6_0    conda-forge
r-th.data                 1.0_10            r35h6115d3f_1    conda-forge
r-zoo                     1.8_7             r35h17f1fa6_0    conda-forge
readline                  8.0                  h0678c8f_2    conda-forge
rpy2                      2.8.6           py27r35hfc83f80_2    conda-forge
scipy                     1.2.1            py27hab3da7d_2    conda-forge
setuptools                44.0.0                   py27_0    conda-forge
singledispatch            3.4.0.3         pyh9f0ad1d_1001    conda-forge
six                       1.15.0             pyh9f0ad1d_0    conda-forge
sqlite                    3.34.0               h17101e1_0    conda-forge
subprocess32              3.5.4            py27h0b31af3_0    conda-forge
tapi                      1100.0.11            h9ce4665_0    conda-forge
tk                        8.6.10               h0419947_1    conda-forge
tktable                   2.10                 h49f0cf7_3    conda-forge
tornado                   5.1.1           py27h1de35cc_1000    conda-forge
traceback2                1.4.0                    py27_0    conda-forge
unittest2                 1.1.0                      py_0    conda-forge
wheel                     0.36.2             pyhd3deb0d_0    conda-forge
xz                        5.2.5                haf1e3a3_1    conda-forge
zlib                      1.2.11            h7795811_1010    conda-forge
zstd                      1.4.8                hf387650_1    conda-forge
(lefse) sbslee@x86_64-apple-darwin13 ~ % 

Finally, can you make sure you are using the exact input files in the tutorial?