ITS Plugin error from itsxpress

hi,all?
When I extract ITS2 sequence, I encounter the following problems: the upstream and downstream sequences do not match. How should I solve them?
Looking forward to a reply

Hello @liubeiping,

Sorry for the delayed response. Would you mind sharing your paired-end-demux.qza file? It seems like there may some problem with the underlying .fastq files.

Thanks.