Issue wile using feature-table group

Hi, I am quite new to use QIIME. I combined 4 groups of samples together to start the analysis. After assign taxonomy, I tried to separate them by "WWTP" using below command.

qiime feature-table group --i-table table.qza --p-axis sample --p-mode sum --m-metadata-file samples-id.txt --m-metadata-column WWTP --o-grouped-table table_WWTP.qza

Then I re-run the assign taxonomy as below,
qiime taxa barplot --i-table table_WWTP.qza --i-taxonomy taxonomy.qza --m-metadata-file samples-id.txt --o-visualization taxa-bar-plots_WWTP.qzv --verbose

The error shows as below:

Plugin error from taxa:

Sample IDs found in the table are missing in the metadata: {'H', 'A', 'P', 'Q'}.

See above for debug info.

It seems after separating, the metadata file was not recognized properly.

Could you please help. Thanks a lot.

Hello and welcome to the forum.
To create a taxonomy barplot with grouped table one need to create a new metadata file with group names as sample id's.

Hi Timur

Thanks, I tried to create a new metadata file as attached one. I put the group name as SampleID(A, H,P,Q). As each group has many samples, there are many duplicates of A, H, P, Q in SampleID. It raises another error saying that Metada IDs must be unique. Could you please advise the format of the new metadata file?


That's right - in collapsed table, each group treated as one "sample", not a group of samples. So, your new metadata should have only one ID per group. Just remove all the duplicates from this new metadata - it needed only for creation of a barplot.

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Yes, it is now solved. Thanks a lot for your prompt reply. Really helps.

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