Issue Using Qiime2R to Create Phyloseq Objects

I have been trying to use the qiime2R package and just can’t get it to work.

I have set my working directory correctly and am able to import my qza files into the workspace for R studio. However, when trying to use the qza_to_phyloseq command (after setting the tmp to an empty folder) I keep getting the following error:

Error in file(con, “r”) : cannot open the connection

I can’t figure out what I am doing wrong and this is so frustrating! Has anyone else had this issue?

Hi @Ellenphant,
See this current topic for troubleshooting this error:

Good luck!