is it possible to train a classifier with multiple genes?

[Gaurav_C (Gaurav Chauhan)]

Hi @SoilRotifer,

Thank you for the help. I have a follow up question. From the links you provided, I learned that we can make a classifier using any of the gene from NCBI database. I would like to know if it is possible to use multiple genes (may be around 50-100 different genes) from NCBI to train ad use a classifier?
I have sequencing data from something like a multiplex PCR (with multiple primers targeting 50-100 different genes)and would like to assign taxonomy to the sequences. Would using multiple genes sequences from different species be a problem for taxonomy assignment?

Thanks,
Gaurav

[Nicholas_Bokulich]

Hi @Gaurav_C ,
Technically you could train such a classifier, but depending on what you want to do with it it might not work well.

For annotating those genes it might work, but for taxonomic classification it would probably not (but you could try!).