Hi,
I have a couple of questions.
-
We did full length 16S+18S sequencing. Our data have both 16S and 18S sequences in the same file. I would like to assign taxonomy to this dataset. I am trying to train a classifier for both 16S and 18S combined and would like to know for the step of extracting reads how do I assign more than 1 primer pair?
Would it look something like this:
qiime feature-classifier extract-reads
–i-sequences 16S+18S.qza
–p-f-primer primerF1 primerF2
–p-r-primer primerR1 primerR2
–o-reads 16S+18S_trimmed.qza -
On the page: Data resources — QIIME 2 2021.11.0 documentation
Naive Bayes classifiers trained on:
- Silva 138 99% OTUs full-length sequences (MD5:
b8609f23e9b17bd4a1321a8971303310
)
Is this classifier for 16S+18S, or just 16S?
Pre-formatted SILVA reference sequence and taxonomy files:
- Silva 138 SSURef NR99 full-length sequences (MD5:
de8886bb2c059b1e8752255d271f3010
)- Silva 138 SSURef NR99 full-length taxonomy (MD5:
f12d5b78bf4b1519721fe52803581c3d
)
Are these files for both 16S and 18S or just 16S?
Thank you!