Invalid value for 'emp-paired-end-sequences.qza'

User Support
1

Have you searched for the problem on the forum? Unfortunately, I couldn't find it.

  • Have you reviewed the QIIME 2 Forum Glossary? Yes
  • Version of QIIME 2 you are running, and how it is installed (e.g. Virtualbox, conda, etc.) - qiime2-2022.2
  • What is the exact command or commands you ran? Copy and paste please.

demultiplexing

qiime demux emp-paired
--m-barcodes-file /metadata/mappingfile_qiime2.txt
--m-barcodes-column BarcodeSequence
--p-rev-comp-mapping-barcodes
--i-seqs emp-paired-end-sequences.qza
--o-per-sample-sequences demux-full.qza
--o-error-correction-details demux-details.qza
--p-no-golay-error-correction
--p-rev-comp-barcodes

  • What is the exact error message, if applicable? If you didn't run the command with the --verbose flag, please re-run and copy-and-paste the results.
    Error message: Invalid value for '--i-seqs': 'emp-paired-end-sequences.qza' is not a QIIME 2 Artifact (.qza)
    We checked for typos, but we couldn't localize them. Is our Qiime2 version ?

Thanks,
Fabiana

2

Hello @fahoffmannsarda,

This error usually happens when you accidentally rename a file that is not a qiime2 artifact to look like one (by adding a .qza extension). Try to see if that or something similar happened.

1 Like
3

Hi Colin,
Thank you very much.
I created the file using this command:

qiime tools import
--type EMPPairedEndSequences
--input-path /sequences/
--output-path emp-paired-end-sequences.qza

and in I had my three not demultiplexed files

barcodes.fastq.gz
forward.fast.gz
reverse.fast.gz

Thanks,
Kindest regards,
Fabiana

1 Like
4

Hello @fahoffmannsarda,

Could you run rm emp-paired-end-sequences.qza (only if you still have the sequences/ folder!) and then rerun the import command and then attach the full terminal log of these steps here?