Invalid metadata

Thank you for the reply!
I actually headed to different direction to figure out this mess…and i finally got to the point where i need to upload my metadata file( not the manifest file which it worked), and im having very similar error as the manifest file…

There was an issue with loading the file vagus_map.csv as metadata:

Found unrecognized ID column name ‘sample-id,Time,Diet’ while searching for header. The first column name in the header defines the ID column, and must be one of these values:

Case-insensitive: ‘feature id’, ‘feature-id’, ‘featureid’, ‘id’, ‘sample id’, ‘sample-id’, 'sampleid’

Case-sensitive: ‘#OTU ID’, ‘#OTUID’, ‘#Sample ID’, ‘#SampleID’, 'sample_name’

There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org

Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2019.4/tutorials/metadata/

I validated my file via Keemi and i used the UTD-8 ver of csv file?

Hi! I suppose you should save you metadata file as metadata.tsv, not metadata.csv format and it should work

I have done trying the tsv file,
But this is what i would get…

There was an issue with loading the file vagus_map.tsv as metadata:

Metadata file must be encoded as UTF-8 or ASCII, found UTF-16. If this file is from Microsoft Excel, save as a plain text file, not 'UTF-16 Unicode’

There may be more errors present in the metadata file. To get a full report, sample/feature metadata files can be validated with Keemei: https://keemei.qiime2.org

Find details on QIIME 2 metadata requirements here: https://docs.qiime2.org/2019.4/tutorials/metadata/

I also have tried txt file but still an error…

Ok. Just try next steps :slight_smile:

  1. Open Google spread sheets
  2. Install keemei plugin
  3. Copy and paste all data
  4. Check with keemei
  5. Download as tsv file.

This steps usually work without errors.

3 Likes

Thank you!!! it worked.
I did not know about exporting into tsv file from keemei.

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