Hi So I haved tried multiple things to get it right but for some reason, it does not work... witht he error msg that i do not know what to do. (qiime2-2019.4) Jiyoungs-MacBook-Pro:Vagus_fastq Sammy$ qiime tools import --type 'SampleData[JoinedSequencesWithQuality]' --input-path 16Smanifest.csv --ou…

Hi @Sammi ! I don't have a ton of experience with this import format, but you may have too many columns in your header. As the error message says, the command expects: [image] Sammi: [‘sample-id’, ‘absolute-filepath’, ‘direction’] Perhaps you are using a complete metadata file, instead of th…

Thank you Chris! So I did try the simpler ver. of the manifest file only with sample id, path, direction. And I got this. There was a problem importing 16Smanifest1.csv: 16Smanifest1.csv is not a(n) SingleEndFastqManifestPhred33 file: Found header on line 1 with the following labels: ['sample_i…

Ah, you have an underscore for sample_id, but it expects a dash sample-id.

Thank you that was the problem! And Now I am having another trouble but i think i can handle it! :slight_smile:

So follow up with this, I get another problem which it says (qiime2-2019.4) Jiyoungs-MacBook-Pro:Vagus_fastq Sammy$ qiime tools import --type 'SampleData[SequencesWithQuality]' --input-path 16Smanifest_TJK.csv --output-path manifest.qza --input-format SingleEndFastqManifestPhred33 There was a prob…

[image] Sammi: (/Users/Sammy/Desktop/Vagus_fastq/vagus_fastq/BHF7.fastq Can you please run the following command and share the results? ls /Users/Sammy/Desktop/Vagus_fastq/vagus_fastq/BHF7.fastq

ls: /Users/Sammy/Desktop/Vagus_fastq/vagus_fastq/BHF7.fastq: No such file or directory This is what i get when i ran the command... I dont understand it, the file is in the folder though?

Hi @Sammi ! Please double-check the spelling and location of the file --- ls isn't lying when it says it can't find that file exactly as specified. Maybe you could share a screenshot of the folder with your sequences in it?

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Error msg from importing the data with the manifest csv,

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