I have made my meta-data, and manifest and validated them. Iv saved visualization. I used the command LS and it shows my sample I’d, forward - absolute - filepath
Reverse-absolute-filepath and then my samples. When I am typing the command
–output-path paired-end-demux. qza
I get an error
There is a problem importing Manifest 2. tsv
manifest2. Tsv is not a (n) PairedEndManifestPhred33v2 file
Filepath on line 1 and colum “forward-absoluts-filepath” could not be found for “Sample-1”
There is a typo here, it should be “forward-absolute-filepath” (note the s->e in “absolute”).
Edit, just realized that you manually wrote out the error message (in the future please copy and paste). Ignore the typo suggestion above. The actual error is that your forward read for “Sample-1” couldn’t be found - that means there is a typo in the filepath you have provided. Please carefully double-check.
I have rewritten the manifest - I have tried both of these commands,qiime tools import
I think i have done it.
My internet on the mountains is so slow- it took hours to complete the 15 samples.
Thanks for all your help.
I am now in the process of denoising. Looking at my visuals its pretty good