I have a problem in importing the data using manifest

[ari_sh70 (Armin)]

Hello all,

I have a problem in importing the data using manifest which I actually don't know what the problem is but I really appreciate it if you can help me out, please.
I am trying to import the paired-end reads in qiime2-2020.8 using the following commands:
qiime tools import
--type 'SampleData[PairedEndSequenceWithQuality]'
--input-path import.txt
--output-path paired_end_demux.qza
--input-format PairedEndFastqManifestPhred32V2

and the error is the following:

Is it possible if I import the reads using Casava paired-end reads method that is has explained in the tutorial?

This is the file that I am trying to import:
import.txt (7.9 KB)

Thank you

[the_dummy]

Hey there,

You have a typo, PairedEndFastqManifestPhred32V2 should be PairedEndFastqManifestPhred33V2.

Good luck!

[ari_sh70 (Armin)]

@the_dummy Thanks very much for your answer.
I have corrected that error but now it tells me that "It does not have a compatible format"

The absolute file path that we are providing should be the path of our reads, no?

Thanks again

[the_dummy]

import-manifest.tsv (178 Bytes)

This is the manifest file format I use to import data. Seems like you are missing /home/username before /Documents in your paths. Could you check if it is /Documents right away? If not, that is the error. You could use pwd in your directory to check the absolute path.

Glad to be helpful.

[ari_sh70 (Armin)]

Thanks for your reply.

Sorry, in the file that i uploaded it wasn’t written the /home/username directories before /Documents but then I corrected but I still got the same error. I do not know why.

[the_dummy]

Well, I have spotted another typo

Format should be SampleData[PairedEndSequencesWithQuality]. You forgot the “s” after “Sequence”.

It would have been better if error message was a bit more clear.

[ari_sh70 (Armin)]

Thanks very much @the_dummy It worked.