Importing manifest file

Hi,
Please I working in Qiime 2 (2018.4), I had a issue on importing manifest file saying
An unexpected error has occurred:
** A path specified in the manifest does not exist or is not accessible: /home/qiime2/Downloads/MI.M00833_0684.001.BioOHT_20rc.20NM_R1.fastq.qz**

Here an example of my manifest file : sample-id,absolute-filepath,direction
20NM,$PWD/MI.M00833_0684.001.BioOHT_20rc.20NM_R1.fastq.qz,forward
21NM,$PWD/MI.M00833_0684.001.BioOHT_21rc.21NM_R1.fastq.qz,forward
22NM,$PWD/MI.M00833_0684.001.BioOHT_22rc.22NM_R1.fastq.qz,forward
23NM,$PWD/MI.M00833_0684.001.BioOHT_23rc.23NM_R1.fastq.qz,forward
24NM,$PWD/MI.M00833_0684.001.BioOHT_24rc.24NM_R1.fastq.qz,forward
25NM,$PWD/MI.M00833_0684.001.BioOHT_25rc.25NM_R1.fastq.qz,forward
26NM,$PWD/MI.M00833_0684.001.BioOHT_26rc.26NM_R1.fastq.qz,forward
27NM,$PWD/MI.M00833_0684.001.BioOHT_27rc.27NM_R1.fastq.qz,forward
20NM,$PWD/MI.M00833_0684.001.BioOHT_20rc.20NM_R2.fastq.qz,reverse
21NM,$PWD/MI.M00833_0684.001.BioOHT_21rc.21NM_R2.fastq.qz,reverse
22NM,$PWD/MI.M00833_0684.001.BioOHT_22rc.22NM_R2.fastq.qz,reverse
23NM,$PWD/MI.M00833_0684.001.BioOHT_23rc.23NM_R2.fastq.qz,reverse
24NM,$PWD/MI.M00833_0684.001.BioOHT_24rc.24NM_R2.fastq.qz,reverse
25NM,$PWD/MI.M00833_0684.001.BioOHT_25rc.25NM_R2.fastq.qz,reverse
26NM,$PWD/MI.M00833_0684.001.BioOHT_26rc.26NM_R2.fastq.qz,reverse
27NM,$PWD/MI.M00833_0684.001.BioOHT_27rc.27NM_R2.fastq.qz,reverse

PWD in my case is home/qiime2/Downloads.
Please any help wiil be appreciated
Thnaks in advance

Hi,
I need your help in importing manifest file.
I had the message below:

An unexpected error has occurred:

** A path specified in the manifest does not exist or is not accessible: /home/qiime2/Downloads/MI.M00833_0684.001.BioOHT_20rc.20NM_R1.fastq.qz**
strong text
This is my manifest file:
manifest.txt (7.1 KB)
Please let me know any suggestions that can help solve the problem
Thanks in advance
Nouha :slight_smile:

Hey there @Nouha!

QIIME 2 was not able to access a file at the location /home/qiime2/Downloads/MI.M00833_0684.001.BioOHT_20rc.20NM_R1.fastq.qz --- does the file exist? If it does, do you have permission to read it?

We can double check - can you run the follow command and provide the full output:

ls -lah /home/qiime2/Downloads/

Thanks! :qiime2:

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