Hi,
Please I working in Qiime 2 (2018.4), I had a issue on importing manifest file saying
An unexpected error has occurred:
** A path specified in the manifest does not exist or is not accessible: /home/qiime2/Downloads/MI.M00833_0684.001.BioOHT_20rc.20NM_R1.fastq.qz**
Here an example of my manifest file : sample-id,absolute-filepath,direction
20NM,$PWD/MI.M00833_0684.001.BioOHT_20rc.20NM_R1.fastq.qz,forward
21NM,$PWD/MI.M00833_0684.001.BioOHT_21rc.21NM_R1.fastq.qz,forward
22NM,$PWD/MI.M00833_0684.001.BioOHT_22rc.22NM_R1.fastq.qz,forward
23NM,$PWD/MI.M00833_0684.001.BioOHT_23rc.23NM_R1.fastq.qz,forward
24NM,$PWD/MI.M00833_0684.001.BioOHT_24rc.24NM_R1.fastq.qz,forward
25NM,$PWD/MI.M00833_0684.001.BioOHT_25rc.25NM_R1.fastq.qz,forward
26NM,$PWD/MI.M00833_0684.001.BioOHT_26rc.26NM_R1.fastq.qz,forward
27NM,$PWD/MI.M00833_0684.001.BioOHT_27rc.27NM_R1.fastq.qz,forward
20NM,$PWD/MI.M00833_0684.001.BioOHT_20rc.20NM_R2.fastq.qz,reverse
21NM,$PWD/MI.M00833_0684.001.BioOHT_21rc.21NM_R2.fastq.qz,reverse
22NM,$PWD/MI.M00833_0684.001.BioOHT_22rc.22NM_R2.fastq.qz,reverse
23NM,$PWD/MI.M00833_0684.001.BioOHT_23rc.23NM_R2.fastq.qz,reverse
24NM,$PWD/MI.M00833_0684.001.BioOHT_24rc.24NM_R2.fastq.qz,reverse
25NM,$PWD/MI.M00833_0684.001.BioOHT_25rc.25NM_R2.fastq.qz,reverse
26NM,$PWD/MI.M00833_0684.001.BioOHT_26rc.26NM_R2.fastq.qz,reverse
27NM,$PWD/MI.M00833_0684.001.BioOHT_27rc.27NM_R2.fastq.qz,reverse
PWD in my case is home/qiime2/Downloads.
Please any help wiil be appreciated
Thnaks in advance