Plugin error from vsearch:
Parameter 'sequences' received an argument of type FeatureData[Sequence]. An argument of subtype SampleData[JoinedSequencesWithQuality] | SampleData[SequencesWithQuality] | SampleData[Sequences] is required.
Anybody got an idea about how to get the fearture table, which using fasta file for import?
Well, my import FASTA file was downloaded from the NCBI Nucleotide database, which do not have information about the original and error-corrected barcodes from which this inference was made.
I tried to get the feature table by using the vsearch plugin, but failed.
So, my question is, how to get the feature table from the FeatureData[Sequence] import? Any other plugin or method exist for feature-table buliding if my import file format is 'FeatureData[Sequence]' ?
Sorry I wasn't clear above --- If that file is a QIIME 1-style post-split-libraries output, you should import it as SampleData[Sequences] (as opposed to FeatureData[Sequences], which you showed in a command above). Then, you can use that as input to vsearch dereplicate-sequences and produce a table and rep-seqs. Does that make sense?