Importing demultiplexed data - not casava format

Technical Support
1

I am trying to help a student import demultiplexed data that does not seem to be in casava format as far as I can tell. The demultiplexed data has been broken up into separate files, but the file names are not in the casava format.

Here is a first few entries of on demultiplexed file. The paired-end barcodes are there in the title lines of these fastq files. Is there a tutorial out there for this kind of demultiplexed file? Or a way to make it work with Q2? Thanks!

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2

Hey @Scott_Kelley1 - are the data already demultiplexed (i.e., you have one R1 and one R2 file per sample)? If so, you could try out this little utility which generates fastq manifest files for importing demultiplexed data into QIIME 2.

3

I’ll give it a whirl! Thanks Greg.

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