Importing Data on QIIME2 -barcode file not recognized

[Ksalg (Katia Salgado)]

Hello,

Super new to QIIME2 and would appreciate suggestions on my current issue. I'm trying to import my data and convert the files into qza format. However, when I run the code I receive an error stating that the barcode file cannot be recognized. (Please see screenshot #1) I'm using the same code as in QIIME2 tutorial guide and stored my files (forward.fastq.gz, reverse.fastq.gz, and barcode.fastq) in a folder named "emp-paired-end-sequences".

Since I used QIIME1 to extract the barcodes, I thought that the error may be due to a format issue. However, on the tutorial website, the barcode file has a similar format as mine (please see screenshot #2). I'm wondering if anyone has had a similar issue and has found a way to fix the error? Any suggestions and/or explanations would be appreciate

Thanks

[Ksalg (Katia Salgado)]

Figured it out! I used the wrong "type" I used EMPPairedEndSequences and zipped the barcode file and it worked

[Keegan-Evans]

@Ksalg, I am glad you got it working, let us know if you need anything else!