Does anyone know,
it is necessary to run qiime cutadapt trim-paired before run dada2.
Hello @cjj102664. Welcome to the forums!
Not necessarily!
cutadapt will 'cut adapters' from your input reads.
Some adapters you can easily remove with the dada2 settings like trim_left_f
and trim_left_r
Some sequencing methods are designed so that they do not include adapters so there is nothing to remove.
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Thank you very much for your reply! I understand!
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