if I dont know what primers in my sequencing data,How do I use qiime2

Does anyone know,
it is necessary to run qiime cutadapt trim-paired before run dada2.

Hello @cjj102664. Welcome to the forums! :qiime2:

Not necessarily!

cutadapt will 'cut adapters' from your input reads. :scissors:

Some adapters you can easily remove with the dada2 settings like trim_left_f and trim_left_r

Some sequencing methods are designed so that they do not include adapters so there is nothing to remove.


Thank you very much for your reply! I understand! :grinning:

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