Hi everyone! Over the past year, the lab I work in has started to become more interested in mouse microbiome studies. The goal is to compare our disease models with wild type mice to see if there are differences.
So far my colleagues and I have analyzed data from a few studies and none have shown significant results, which I fear is the result of low sample sizes. One of our studies had two experimental groups with 3 mice each, another had 2 groups of 4, and another had 5 groups of 3-4 each.
Based on my search of the literature, it seems that many mouse microbiome studies have 5-6+ mice per group. And even when browsing this forum, it appears that a lot of researchers are using larger sample sizes than what my lab has done so far. Is this true? For those with experience in these types of studies, what sample sizes do you usually use? Is it reasonable to think that perhaps increasing sample size could lend different results? I know it’s possible that there truly aren’t any significant differences between these disease states and wild type, and that maybe adding a couple more mice to the study wouldn’t make any difference. I’m just trying to decide if it would be worth it to repeat these studies with more mice.
I appreciate any advice or input!! Thank you!