how to train my classifier for V3-V4 16SRNA gene region

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Hi @Cele_Blua,

Training the full-length 16S rRNA gene classifier can take a substantial amount of memory. Often requiring anywhere from 24 - 64 GB of RAM! You can make use of premade classifiers from the data resources page. However, if you do not have the memory to train the classifier, there is a chance you might not have enough memory to use the classifier, at least without modifying some options.

You can...

  1. simply use the classifier to see if the outputs make sense.
  2. use some of the built-in evaluation functionality of RESCRIPt.

-Cheers!
-Mike

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