How to prepare FASTQ file for individual sample after demux using barcodes?


I am trying to upload my 16S sequencing data (paired-end) on NCBI SRA (sequence read archive) and it appears that I need to upload FASTQ data for individual samples after demux using barcodes which I have in trouble in getting (What I have now is forward, reverse and barcode sequence fastq files)

Could you please let me know how to get fastq files for individual samples after demux using barcodes?

Thank you very much in advance for your help!

Hi @scho73,

Use qiime tools export to export the demultiplexed data — this will yield a directory containing per-sample fastqs.

I hope that helps!

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