how to normalize picrust2 predicted results

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Hi QIIME2 team,

I used PICRUSt2 and obtained the EC_metagenome_out/pred_metagenome_unstrat.tsv file. I noticed that the EC abundance values in the output appear to vary with sequencing depth — samples with higher sequencing depth show much higher predicted EC abundances.

Could you please advise on the recommended normalization method for downstream analyses such as PCoA visualization, differential abundance testing between case and control groups?

Thank you for your support!
Best.

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