How to import paired end data from miseq?

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Hello, everyone.

I'm struggling with data importing right now. I'm using qiime2.2024.2 version.

The data is obtained via miseq and the files are in the form of OO-forward.fastq.qz, OO-reverse.fastq.qz.
Barcode is expected to be exist in the files.

I'm not sure what type to use to import the data into the qiime2.
I tried Casava, EMP, SampleData, Multiplexed, and Multiplexed and they all give me an error.

When I used iseq before, it existed as a casava type of file name, but this time it's different...

So I tried to create a manifest file and put it in, but it doesn't work.... What should I do?

I'll also upload the manifest file.

I look forward to your response, thank you.

bioflor-manifest.tsv (10.2 KB)

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Hi @ehdud0505,
I think this import guide should be exactly what you want.

Multiplexed paired-end FASTQ with barcodes in sequence

Note: you may have to rename your files!

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Hi @cherman2,

The data was successfully imported thanks to your advice to rename the file. Thank you so much!

  • Doyoung -
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