how to construct barplot after feature table grouping

[Xio_Lee]

Hi,

Just wondering how to construct barplot after feature-table group. I grouped the table by sampling depth but it popped up error during barplot:

qiime taxa barplot \
--i-table grouped/table_groupby_depth_16s.qza \
--i-taxonomy taxonomy/taxonomy_mcm_16s.qza \
--m-metadata-file ../../metadata/metadata_16s.tsv \
--o-visualization taxonomy/barplot_grouped_mcm_16s.qzv

error msg:
Feature IDs found in the table are missing in the metadata: {'4inch', '8inch', '12inch'}

I am not sure how can I modify the metadata after grouping?

Thanks,

Xiao

[Nicholas_Bokulich]

whenever you group a feature table, the axis you are grouping on (samples or features) adopt the group labels that you are using. In your case, you are grouping on the sample axis (grouping by sample depth, a column in your original metadata file). So you need to create a new metadata file that has sample IDs consisting of the column you used for grouping.

So in your case, you just need this:

sample-id	whatever	metadata	columns	you	desire
4inch	4	inch	sampling	depth	metadata
8inch	8	inch	sampling	depth	metadata
12inch	12	inch	sampling	depth	metadata