We would like to analyze the stools of a certain population before and after the treatment to find out the bacteria that were changed by the treatment.
However, one of the problems with this is that if one person's intestinal bacteria changes dramatically, it may have an effect on the entire population.
(For example, if one person's Lactobacillus increases due to treatment, it may appear that the overall Lactobacillus is increasing even if it is not increasing in other people.
The larger the sample size, the less likely this problem is to occur, but since our sample size is small, we are trying to eliminate it as much as possible.
By using lefse, it seems to have the above problem, but in that case, is it ANCOM?