Hi, Im studying microbial eukaryotic diversity in the Korea.
I prepared reference data this way.
qiime tools import
--type 'FeatureData[Sequence]'
--input-path /Users/lompe/murder/pr2.fasta
--output-path /Users/lompe/murder/pr2.qza
qiime feature-classifier extract-reads
--i-sequences /Users/lompe/Qiimetest/pr2.qza
--p-f-primer CCAGCASCYGCGGTAATTCC
--p-r-primer ACTTTCGTTCTTGATYRA
--o-reads /Users/lompe/Qiimetest/v4_extracts.qza
The problem is how I check the quality score in v4_extracts.qza
This is a error message.
(qiime2-2018.6) LabOfMarineProtistanEcophysioloygy:Qiimetest lompe$ qiime demux summarize \
--i-data v4_extracts.qza
--o-visualization v4_extracts.qzv
Plugin error from demux:
Argument to parameter 'data' is not a subtype of SampleData[JoinedSequencesWithQuality | PairedEndSequencesWithQuality | SequencesWithQuality].
Debug info has been saved to /var/folders/kv/pkjw8_2d2_x9ydbtrbt_ns9c0000gn/T/qiime2-q2cli-err-3xuob14i.log
And I tried make demux.qza by reference data. But I had a no barcode file and metadata.
Please help me, thanks.