How I check the quality score in reference data?

Hi, Im studying microbial eukaryotic diversity in the Korea.

I prepared reference data this way.

qiime tools import
–type ‘FeatureData[Sequence]’
–input-path /Users/lompe/murder/pr2.fasta
–output-path /Users/lompe/murder/pr2.qza

qiime feature-classifier extract-reads
–i-sequences /Users/lompe/Qiimetest/pr2.qza
–p-f-primer CCAGCASCYGCGGTAATTCC
–p-r-primer ACTTTCGTTCTTGATYRA
–o-reads /Users/lompe/Qiimetest/v4_extracts.qza

The problem is how I check the quality score in v4_extracts.qza

This is a error message.

(qiime2-2018.6) LabOfMarineProtistanEcophysioloygy:Qiimetest lompe$ qiime demux summarize \

–i-data v4_extracts.qza
–o-visualization v4_extracts.qzv
Plugin error from demux:

Argument to parameter ‘data’ is not a subtype of SampleData[JoinedSequencesWithQuality | PairedEndSequencesWithQuality | SequencesWithQuality].

Debug info has been saved to /var/folders/kv/pkjw8_2d2_x9ydbtrbt_ns9c0000gn/T/qiime2-q2cli-err-3xuob14i.log

And I tried make demux.qza by reference data. But I had a no barcode file and metadata.

Please help me, thanks.

Hi @Don,
Your input data is fasta format:

fasta format does not contain any quality scores; hence it is imported as FeatureData[Sequence] type. This is not one of the required types for that command (which contain quality scores):

I hope that helps clarify!

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