How cluster ASV with different length in OTU ?

[crusher083 (Valentyn Bezshapkin)]

Hello,

you need to trim your ASV. Depending on how little is minimal length, you can either trim everything to a minimum (that was the case in the American Gut study) or discard sequences, that are too short.
In this thread, OP asks similar question about trimming.

Truncate reads before VSEARCH with pyrosequecing/ion torrent data

First, thanks a lot for all the development in qiime2 and the help provided here, you are amazing people! I need to analyze several 454 and ion-torrent datasets. I would like to use clustering-vsearch-otus first, and it would be great to do the whole analysis in qiime2. I know that there is a straightforward tutorial for this already in qiime2: Clustering sequences into OTUs using q2-vsearch. BUT the tutorial starts with already quality-control data. Short question: ¿how can I quality-control…

Cheers,
V