Help!! With 18S Dada2 'Filtering Error in names(answer)'

[CalvinCornell]

Howdy everyone can I please have some help!!!!!

I am trying to run an analysis for fungal SSU (I think 18S) I have paired reads already organized into their respected samples it is from I believe Sanger/Illuminia 1.9
manifest.tsv (13.6 KB)

I imported then into qiime2 using
qiime tools import --type 'SampleData[PairedEndSequencesWithQuality]' --input-path manifest.tsv --output-path paired_demux.qza --input-format PairedEndFastqManifestPhred33V2

qiime demux summarize --i-data paired_demux.qza --o-visualization paired_demux_vis.qzv
paired_demux_vis.qzv (317.3 KB)

From looking at this file and my manifest file all forward and reverse reads have the same names and same amount of reads

(I didn't do demultiplexing as all my reads are sorted into sample files)

qiime dada2 denoise-paired --i-demultiplexed-seqs paired_demux.qza --p-trunc-len-f 250 --p-trunc-len-r 250 --p-n-threads 0 --o-table table_new.qza --o-representative-sequences rep_seq_new.qza --o-denoising-stats denoise_state_new.qza

(these p-trunc-len are super low as I have been trying to see if adjusting it helps)

However dada2 runs for a few minutes then I get
error_message.txt (4.9 KB)
with one of the things I notice is 2) Filtering Error in names(answer) <- names1 :
'names' attribute [136] must be the same length as the vector [73]

[colinvwood (Colin Wood)]

Hello @CalvinCornell,

I found this pertinent discussion on dada2's GitHub. What version of qiime2 are you using?

[CalvinCornell]

I thank you so very much for getting back to me and your help in providing this link to this original post and I found the same solution in that I was requesting/using too many threads once I reduced the number dada2 worked great