Hi, thank you all so much for your continued support and help!!!
I am having trouble determining how to make my metadata mapping file, for paired-end reads. The company used dual-indexing Nextera Adapters and didn’t specify the Golay barcodes named as such, just the i5 and i7 index sequences (the data came to us demultiplexed and trimmed already). I wasn’t sure how to incorporate Nextera (or what barcode to use) into my mapping file (draft below):
#SampleID BarcodeSequence LinkerPrimerSequence ReverseLinkerPrimerSequence
5.105-G ? GTGTGYCAGCMGCCGCGGTAA CCGGACTACNVGGGTWTCTAAT
5.111-B ? GTGTGYCAGCMGCCGCGGTAA CCGGACTACNVGGGTWTCTAAT
The Nextera info would be -
#Sample ID Index 1 [i7] Index 1 sequence Index 2 [i5] Index 2 sequence
5.105-G N723 TAGCGCTC S515 TTCTAGCT
5.111-B N702 CGTACTAG S513 TCGACTAG
Any help would be much appreciated!