I’m filtering merged sequences, trying to set a specific cutoff for the minimum length of the sequence length that I get as an output but I’m not getting the results I’m looking for. This is what I’m trying:
qiime quality-filter q-score \
My desired seq length is a minimum of 400, I’ve played with different “–p-min-length-fraction” parameters (0, 0.9, 0.97) but I don’t see a clear change. The mean length of my seq is 445.02 with a standard deviation of 26.82.